The Journal of Veterinary Medical Science

Vol.78, No.11 November 2016

Vol.78, No.11 November 2016
Method for isolating pure bovine gonadotrophs from anterior pituitary using magnetic nanoparticles and anti-gonadotropin-releasing hormone receptor antibody
Kiran PANDEY et al. (pp.1699–1702)

Gonadotrophs in the anterior pituitary (AP) are important cells; however, the AP has a heterogeneous cell population, and gonadotrophs constitute only 10 to 15% of all cells in the AP and are scattered among other cell types. Pandey et al. developed a novel and simple method for the rapid simultaneous purification of bovine gonadotrophs from heterogeneous AP cell mixture. Fluorescent immunohistochemistry confirmed that all of the purified cells expressed GnRHR (light blue) and LH (red); Nuclear DNA was counterstained with DAPI (dark blue), and cell morphology was visualized by differential interference contrast (DIC) imaging. The MERGE is an overlay of the four panels.

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